Products
PCR Reagents
PCR Reagents
TetraPrime™
Conventional PCR
Master Mixes
DESCRIPTION
Every TetraPrime™ Master Mix features our in-house TetraZyme™ Polymerase in a convenient, ready-to-use format. Carrying the superior dNTP binding and primer retention of SGME-engineered TetraZyme™ into every reaction, it delivers stable, efficient DNA amplification every time.
APPLICATION
Conventional PCR / One-step RT-PCR
PERFORMANCE
1) Conventional PCR Master Mix
Figure 1. Hot-start PCR performance comparison using lambda DNA (long PCR)
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1: Hot Start / 2: Hot Start Elite / 3: Hot Start Supreme / 4: Leading global brand
TetraPrime™ Hot Start Master Mix delivers superior amplification performance compared with a leading global brand’s PCR reagent.
ORDERING INFORMATION
Conventional PCR
| Cat. No. | Product Name | Pack Size |
|---|---|---|
| 111101 | TetraPrime™ PCR 2x Master Mix | 5×1mL (500rxn) |
| 111102 | TetraPrime™ Elite PCR 2x Master Mix | 5×1mL (500rxn) |
| 111103 | TetraPrime™ Hot Start PCR 2x Master Mix | 5×1mL (500rxn) |
| 111104 | TetraPrime™ Hot Start Elite PCR 2x Master Mix | 5×1mL (500rxn) |
| 111105 | TetraPrime™ Hot Start Supreme PCR 2x Master Mix | 5×1mL (500rxn) |
| 111106 | TetraPrime™ High Fidelity PCR 2x Master Mix | 5×1mL (500rxn) |
| 111107 | TetraPrime™ One-step RT-PCR 2x Master Mix | 5×1mL (500rxn) |
TetraPrime™
Real-time PCR
Master Mixes
DESCRIPTION
The full power of TetraZyme™, in every reaction. Built on the strong signal intensity of TetraZyme™, TetraPrime™ SYBR Green and Probe Master Mixes deliver higher fluorescence, earlier Ct values and sharper melt peaks than conventional Taq-based master mixes.
APPLICATION
Real-time PCR / One-step qPCR
PERFORMANCE
2) Real-time PCR Master Mix
Figure 2. Real-time PCR (SYBR Green) performance comparison using GAPDH and Verotoxin 1
Real-time PCR Amplification
PCR Conditions & Targets
Targets:
GAPDH (440 bp)
Verotoxin1 (725 bp)
PCR conditions:
95°C 3min / 95°C 10sec / 60°C 20sec (45cycles)
Real-time PCR Melt Curve
Real-time PCR Melt Peak
Compared with a typical Taq-based master mix, TetraPrime™ SYBR Green Master Mix delivers higher fluorescence intensity, earlier Ct values and more clearly defined melt peaks.
It is ideal for quantitative gene expression analysis, viral detection, multiplex detection and high-throughput clinical testing.
ORDERING INFORMATION
Real-time PCR
| Cat. No. | Product Name | Pack Size |
|---|---|---|
| 112101 | TetraPrime™ Real-time PCR 2x Probe Master Mix | 5×1mL (500rxn) |
| 112102 | TetraPrime™ Real-time PCR 2x Master Mix (SYBR Green) | 5×1mL (500rxn) |
| 112103 | TetraPrime™ Real-time PCR 2x Master Mix (SYBR and ROX) | 5×1mL (500rxn) |
| 112106 | TetraPrime™ One-step qRT-PCR 2x Probe Master Mix | 5×1mL (500rxn) |
| 112107 | TetraPrime™ One-step qRT-PCR 2x Probe Master Mix (UDG) | 5×1mL (500rxn) |
| 112108 | TetraPrime™ One-step qRT-PCR 2x Master Mix (SYBR Green) | 5×1mL (500rxn) |
| 112109 | TetraPrime™ One-step qRT-PCR 2x Master Mix (SYBR Green and ROX) | 5×1mL (500rxn) |
TetraScript™
cDNA Synthesis Kit
DESCRIPTION
TetraScript™ delivers consistent, highly reliable first-strand cDNA synthesis for a wide range of downstream applications, including RT-PCR, qRT-PCR and NGS. Its performance has been validated on human total RNA across four housekeeping genes (GAPDH, HuPO, PGK and Ubc).
APPLICATION
cDNA Synthesis
PERFORMANCE
3) cDNA Synthesis
Figure 3. cDNA Synthesis Performance and Downstream qPCR Validation Using Human RNA
qPCR Amplification Curve
- Targets :
- GAPDH / HuPO / PGK / Ubc
- PCR conditions :
- 95°C 3min / 95°C 10sec / 60°C 20sec
(45 cycles)
- * Template :
- cDNA (1/10 dilution of synthesis reaction)
Gel electrophoresis
- Targets :
- GAPDH / HuPO / PGK / Ubc
- PCR conditions :
- 95°C 5min / 94°C 20sec / 68°C 1min /
72°C 1min (35 cycles)
- * Template :
- cDNA (1/10 dilution of synthesis reaction)
TetraScript™ cDNA Synthesis Kit provides highly efficient first-strand cDNA synthesis, enabling stable, reliable amplification across a broad range of targets.
Using human total RNA as the template, we evaluated the cDNA synthesis efficiency and downstream qPCR performance of the TetraScript™ cDNA Synthesis Kit (Cat. No. 113101). Conventional RT-PCR (gel electrophoresis) and qPCR targeting four housekeeping genes (GAPDH, HuPO, PGK and Ubc) confirmed consistent, robust amplification across all targets.
ORDERING INFORMATION
cDNA Synthesis
| Cat. No. | Product Name | Pack Size |
|---|---|---|
| 113101 | TetraScript™ cDNA Synthesis Kit | 2.5 mL (500rxn) |